简易的多重
PCR文库构建技术
SLIMamp® 多重PCR文库制备技术
SLIMamp
(Stem-Loop Inhibition Mediated amplification)
技术可以在单管内通过多重PCR反应实现多个基因目标区域的扩增,利用独特的引物设计,重叠区域的扩增子形成一个茎环(stem loop)结构,阻止了引物的退火结合,进而抑制重叠区域扩增子的进一步扩增。
使用SLIMamp技术构建文库简单高效,可以在8小时内完成建库,最低投入量仅需2.5ng,并具有较高的比对率和中靶率。
使用SLIMamp技术构建文库简单高效,可以在8小时内完成建库,最低投入量仅需2.5ng,并具有较高的比对率和中靶率。
SLIMamp® 技术优势
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单天完成建库,操作时间低至2小时
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低起始量样本高效建库
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